In the field of biological research, particularly in medicine and biotechnology, there is a growing emphasis on sequence analysis. This involves analyzing large amounts of targeted and whole genome data to gain insights into various biological phenomena. To facilitate this process, we introduce The Bioconductor project, which serves as a comprehensive platform for sharing software tools, workflows, and datasets specifically designed for the analysis and comprehension of genomic data. Bioconductor offers a wide range of resources that are easily accessible to researchers. It is an open software resource that has been developed by a community of experts in the field. By participating in this course, you will gain proficiency in utilizing essential Bioconductor packages and become familiar with its infrastructure and the built-in datasets it provides. Throughout the course, you will have the opportunity to work with real datasets from different species, using tools such as BSgenome, Biostrings, IRanges, GenomicRanges, TxDB, ShortRead, and Rqc. This hands-on experience will undoubtedly be invaluable in enhancing your understanding and skills in genomic data analysis.